TY  - JOUR
A1  - Hammond, Andrew
A1  - Pollegioni, Paola
A1  - Persampieri, Tania
A1  - North, Ace
A1  - Minuz, Roxana
A1  - Trusso, Alessandro
A1  - Bucci, Alessandro
A1  - Kyrou, Kyros
A1  - Morianou, Ioanna
A1  - Simoni, Alekos
A1  - Nolan, Tony
A1  - Müller, Ruth
A1  - Crisanti, Andrea
T1  - Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field
T2  - Nature Communications
N2  - CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles gambiae effectively suppressed the reproductive capability of mosquito populations reared in small laboratory cages. To bridge the gap between laboratory and the field, this gene-drive technology must be challenged with vector ecology. Here we report the suppressive activity of the gene-drive in age-structured An. gambiae populations in large indoor cages that permit complex feeding and reproductive behaviours. The gene-drive element spreads rapidly through the populations, fully supresses the population within one year and without selecting for resistance to the gene drive. Approximate Bayesian computation allowed retrospective inference of life-history parameters from the large cages and a more accurate prediction of gene-drive behaviour under more ecologically-relevant settings. Generating data to bridge laboratory and field studies for invasive technologies is challenging. Our study represents a paradigm for the stepwise and sound development of vector control tools based on gene-drive.
KW  - Behavioural ecology
KW  - Environmental biotechnology
KW  - Population dynamics
KW  - Synthetic biology
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63628
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-636286
SN  - 2041-1723
N1  - Supported by a grant from the Bill & Melinda Gates Foundation and from the Open Philanthropy Project Fund, an advised fund of Silicon Valley Community Foundation.
VL  - 12.2021
IS  - art. 4589
SP  - 1
EP  - 9
PB  - Nature Publishing Group UK
CY  - [London]
ER  -