TY  - JOUR
A1  - Kubatova, Nina
A1  - Mao, Jiafei
A1  - Eckert, Carl Elias
A1  - Saxena, Krishna
A1  - Gande, Santosh Lakshmi
A1  - Wachtveitl, Josef
A1  - Glaubitz, Clemens
A1  - Schwalbe, Harald
T1  - Light dynamics of the retinal-disease-relevant G90D bovine rhodopsin mutant
T2  - Angewandte Chemie
N2  - The RHO gene encodes the G-protein-coupled receptor (GPCR) rhodopsin. Numerous mutations associated with impaired visual cycle have been reported; the G90D mutation leads to a constitutively active mutant form of rhodopsin that causes CSNB disease. We report on the structural investigation of the retinal configuration and conformation in the binding pocket in the dark and light-activated state by solution and MAS-NMR spectroscopy. We found two long-lived dark states for the G90D mutant with the 11-cis retinal bound as Schiff base in both populations. The second minor population in the dark state is attributed to a slight shift in conformation of the covalently bound 11-cis retinal caused by the mutation-induced distortion on the salt bridge formation in the binding pocket. Time-resolved UV/Vis spectroscopy was used to monitor the functional dynamics of the G90D mutant rhodopsin for all relevant time scales of the photocycle. The G90D mutant retains its conformational heterogeneity during the photocycle.
KW  - G-protein-coupled receptors
KW  - NMR spectroscopy
KW  - retinal
KW  - rhodopsin
KW  - UV/Vis spectroscopy
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63842
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638428
SN  - 1521-3773
N1  - This workwas supported by the DFG (SFB807). Work at BMRZ issupported by the state of Hesse.Open access funding enabledand organized by Projekt DEAL.
N1  - A previous version of this manuscript has been published as part of a thesis (http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52605).
VL  - 59
IS  - 36
SP  - 15656
EP  - 15664
PB  - Wiley-VCH
CY  - Weinheim
ER  -