TY  - JOUR
A1  - Kahl, Günter
A1  - Lange, Horst
A1  - Rosenstock, Günter
T1  - Regulation glykolytischen Umsatzes durch Synthese und Abbau von Enzymen
T1  - Regulation of glycolysis by the synthesis and degradation of enzymes
T2  - Zeitschrift für Naturforschung, B
N2  - Differential derepression of the genome of potato tuber cells causes the onset of a vigorous metabolic activity, which is initiated by rapid synthesis of different RNA species, various proteins and phospholipids. Consequently enhanced respiration and the build up of cell compartments such as ribosomes and mitochondria as well as the performance of cell divisions and suberization of new-formed cell walls occur. Although there is an activation of metabolism in general with a concomitant rise in concentration of most glycolytic metabolites — as was proved for fructose-1.6-diphosphate, dihydroxyacetone, glyceraldehade-3-phosphate, phosphoenolepyruvate and pyruvate — the activities of the corresponding enzymes do not reflect these uniform metabolic changes. Aldolase and in a pronounced manner enolase and glutamate — pyruvate — transaminase lower their activities suddenly after derepression. The activity of triosephosphateisomerase remains constant. In contrast phosphoglyceromutase, pyruvate kinase and to a lower extent malic enzyme enhance their action during the same time.
Without doubt, differential lowering and enhancing the activity of glycolytic chain constituents at the same time is an important regulatory mechanism of the cell. The activation represents de novo synthesis of the protein concerned whereas the inactivation depends largely on protein synthesis. This is clearly shown by experiments with inhibitors of protein synthesis.
It is proposed that this differential synthesis and degradation represent a “long-time-regulation” of enzymatic activity of the cell in contrast to the known “short-time-regulation” by feedback or competition.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74574
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-745744
SN  - 1865-7117
SN  - 0932-0776
VL  - 24.1969
IS  - 12
SP  - 1544
EP  - 1549
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -