TY - JOUR A1 - Kahl, Günter T1 - De novo synthesis of glucose-6-phosphate- (E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (E.C. 1.1.1.44) in plant storage tissue slices T2 - Zeitschrift für Naturforschung, C N2 - Resting potato tuber tissue possesses only faint activity of the two dehydrogenases of the oxidative pentose phosphate cycle, glucose-6-phosphate- and 6-phosphogluconate dehydrogenase. Slicing of the tissue, however, greatly enhances the action of both enzymes. The slicing-induced increase in activity is a consequence of intensified action of at least 5 glucose-6-phosphate dehydrogenase isozymes and a more differentiated activation/inactivation of seven 6-phosphogluconate dehydrogenase isozymes. Using density labelling and isopycnic equilibrium centrifugation it could be demonstrated, that the bulk of both enzymes appearing after slicing the tissue is the result of de novo synthesis rather than activation of pre-existing proenzymes. KW - Glucose-6-phosphate- and 6-phosphogluconate dehydrogenase KW - Isozyme Pattern KW - de novo Synthesis KW - Potato Tuber Slices Y1 - 2014 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/71820 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-718208 SN - 0939-5075 SN - 1865-7125 VL - 29.1974 IS - 11-12 SP - 700 EP - 704 PB - Verlag der Zeitschrift für Naturforschung CY - Tübingen ER -