Effect of sonic hedgehog/β-TCP composites on bone healing within the critical-sized rat femoral defect

  • The creation of entirely synthetically derived bone substitute materials which are as effective as autologous bone grafts is desirable. Osteogenesis involves the concerted action of several proteins within a signaling cascade. Hedgehog proteins act upstream of this cascade, inducing the expression of various bone morphogenetic proteins (BMPs) and promoting physiological bone healing. Therefore, the hypothesis that hedgehog signaling in bone defects improves bone healing more than BMP signaling alone was tested. Recombinant N-terminal sonic hedgehog protein (N-SHh), BMP-2 or a combination of the two was added to β-tricalcium phosphate (β-TCP) and 5-mm femoral midshaft defects in nude rats were filled with these composites. The defects were stabilized with mini-plates. After eight weeks, the animals were sacrificed and the femora were explanted. The radiological evaluation was followed by a three-point bending test and histological examination. BMP-2/β-TCP composites showed a trend of increased stiffness compared with the controls (β-TCP without protein). N-SHh/β-TCP composites had lower stiffness compared with the control group and the N-SHh/BMP-2/β-TCP composites also had lower average stiffness compared with the controls (all not significant). Histomorphometry, however, revealed abundant cartilage and bone core formation in the N-SHh-composite groups. The sum of the new cartilage and bone was highest in the combination group N-SHh/BMP-2 (not significant). The addition of N-SHh to bone substitute materials appears to delay bone healing at the applied concentration and observation time but also showed a trend for higher amounts of ossifying cartilage.

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Author:Jörg Warzecha, Caroline Seebach, Armin Niklas Flinspach, Florian Wenger, Dirk Henrich, Ingo MarziORCiDGND
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/23596469
Parent Title (English):Experimental and Therapeutic Medicine
Publisher:Spandidos Publ.
Place of publication:Athens
Document Type:Article
Year of Completion:2013
Date of first Publication:2013/01/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/05/10
Tag:bone morphogenetic protein-2; bone substitute material; rat femur defect; sonic hedgehog; three point bending test; tricalcium phosphate
Page Number:5
First Page:1035
Last Page:1039
Copyright © 2013, Spandidos Publications. This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0