Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

  • The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersensitivity reactions, and fibrosis. Here we show that artificial particles of specific sizes affect immune cell recruitment as tested in a dermal air pouch model in mice. In addition, we demonstrate that the composition of nanoparticles may influence immune cell recruitment in vivo. Aside from biophysical characterizations in terms of hydrodynamic diameter, zeta potential, concentration, and atomic concentration of metals, we show that - after first-line in vitro assays - characterization of cellular and molecular effects by dermal air pouch analysis is straightforward and should be included in the quality control of nanoparticles. We demonstrate this for innate immunological effects such as neutrophil recruitment and the production of immune-modulating matrix metalloproteases such as MMP-9; we propose the use of air pouch leukocytosis analysis as a future standard assay.

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Author:Jennifer Vandooren, Nele Berghmans, Chris Dillen, Ilse Van Aelst, Isabelle Ronsse, Liron Limor Israel, Ina Rosenberger, Jörg KreuterGND, Jean-Paul Lellouche, Shulamit Michaeli, Erica LocatelliORCiDGND, Mauro Comes Franchini, Miren K. Aiertza, Laura Sánchez-Abella, Iraida Loinaz, Dylan R. Edwards, Louis Shenkman, Ghislain Opdenakker
Pubmed Id:
Parent Title (English):International journal of nanomedicine
Publisher:DOVE Medical Press
Place of publication:Albany, Auckland [u.a.]
Document Type:Article
Date of Publication (online):2013/12/13
Date of first Publication:2013/12/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/06/18
Tag:air pouch; biocompatibility; immunology; nanoparticles; toxicity
Page Number:12
First Page:4745
Last Page:4756
© 2013 Vandooren et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0