Species differences in bacterial NhaA Na+/H+ exchangers

  • Bacteria have adapted their NhaA Na(+)/H(+) exchangers responsible for salt homeostasis to their different habitats. We present an electrophysiological and kinetic analysis of NhaA from Helicobacter pylori and compare it to the previously investigated exchangers from Escherichia coli and Salmonella typhimurium. Properties of all three transporters are described by a simple model using a single binding site for H(+) and Na(+). We show that H.pylori NhaA only has a small acidic shift of its pH-dependent activity profile compared to the other transporters and discuss why a more drastic change in its pH activity profile is not physiologically required.

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Author:Octavian CălinescuORCiDGND, Eva Danner, Marc Böhm, Carola Hunte, Klaus FendlerORCiDGND
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25017436
Parent Title (English):FEBS letters
Place of publication:Amsterdam [u. a.]
Document Type:Article
Date of Publication (online):2014/10/23
Date of first Publication:2014/08/25
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/10/23
Tag:Cation/proton antiport; Escherichia coli; Helicobacter pylori; NhaA; Salmonella typhimurium; Solid supported membrane
Page Number:7
First Page:3111
Last Page:3116
(c) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0