Visualization of newly synthesized neuronal RNA in vitro and in vivo using click-chemistry

  • The neuronal transcriptome changes dynamically to adapt to stimuli from the extracellular and intracellular environment. In this study, we adapted for the first time a click chemistry technique to label the newly synthesized RNA in cultured hippocampal neurons and intact larval zebrafish brain. Ethynyl uridine (EU) was incorporated into neuronal RNA in a time- and concentration-dependent manner. Newly synthesized RNA granules observed throughout the dendrites were colocalized with mRNA and rRNA markers. In zebrafish larvae, the application of EU to the swim water resulted in uptake and labeling throughout the brain. Using a GABA receptor antagonist, PTZ (pentylenetetrazol), to elevate neuronal activity, we demonstrate that newly transcribed RNA signal increased in specific regions involved in neurogenesis.

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Author:Güney Akbalik, Kasper Langebeck-Jensen, Georgi Tushev, Sivakumar Sambandan, Jennifer Rinne, Irina Epstein, Ivan Cajigas, Irena Vlatkovic, Erin Schuman
Pubmed Id:
Parent Title (English):RNA Biology
Publisher:Taylor and Francis
Document Type:Article
Date of Publication (online):2016/11/01
Year of first Publication:2016
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/11/16
Tag:Nascent RNA; PABP; RNA in zebrafish brain; Y10B; neuronal RNA; newly synthesized RNA; visualization of RNA
Page Number:9
First Page:20
Last Page:28
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Hirnforschung
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0