Visualization of newly synthesized neuronal RNA in vitro and in vivo using click-chemistry

  • The neuronal transcriptome changes dynamically to adapt to stimuli from the extracellular and intracellular environment. In this study, we adapted for the first time a click chemistry technique to label the newly synthesized RNA in cultured hippocampal neurons and intact larval zebrafish brain. Ethynyl uridine (EU) was incorporated into neuronal RNA in a time- and concentration-dependent manner. Newly synthesized RNA granules observed throughout the dendrites were colocalized with mRNA and rRNA markers. In zebrafish larvae, the application of EU to the swim water resulted in uptake and labeling throughout the brain. Using a GABA receptor antagonist, PTZ (pentylenetetrazol), to elevate neuronal activity, we demonstrate that newly transcribed RNA signal increased in specific regions involved in neurogenesis.

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Author:Güney Akbalik, Kasper Langebeck-Jensen, Georgi Tushev, Sivakumar Sambandan, Jennifer Rinne, Irina Epstein, Ivan Cajigas, Irena Vlatkovic, Erin Schuman
URN:urn:nbn:de:hebis:30:3-439249
DOI:https://doi.org/10.1080/15476286.2016.1251541
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/27801616
Parent Title (English):RNA Biology
Publisher:Taylor and Francis
Document Type:Article
Language:English
Date of Publication (online):2016/11/01
Year of first Publication:2016
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/11/16
Tag:Nascent RNA; PABP; RNA in zebrafish brain; Y10B; neuronal RNA; newly synthesized RNA; visualization of RNA
Volume:14
Issue:1
Page Number:9
First Page:20
Last Page:28
Note:
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
HeBIS-PPN:430463057
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Hirnforschung
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0