Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo‐tomography

  • We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
Author:Vicki A. M. Gold, Piotr Chroscicki, Piotr Bragoszewski, Agnieszka Chacinska
Pubmed Id:
Parent Title (English):EMBO reports
Publisher:EMBO Press ; Wiley
Place of publication:Heidelberg ; Hoboken, NJ [u. a.]
Document Type:Article
Year of Completion:2017
Date of first Publication:2017/08/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/06/07
Tag:TOM complex; electron cryo-tomography; mitochondrial protein import; ribosomes; translation
Page Number:15
First Page:1786
Last Page:1800
License: This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Institutes:Biowissenschaften / Biowissenschaften
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 4.0