Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows

  • Mitochondrial ATP synthases form dimers, which assemble into long ribbons at the rims of the inner membrane cristae. We reconstituted detergent-purified mitochondrial ATP synthase dimers from the green algae Polytomella sp. and the yeast Yarrowia lipolytica into liposomes and examined them by electron cryotomography. Tomographic volumes revealed that ATP synthase dimers from both species self-assemble into rows and bend the lipid bilayer locally. The dimer rows and the induced degree of membrane curvature closely resemble those in the inner membrane cristae. Monomers of mitochondrial ATP synthase reconstituted into liposomes do not bend membrane visibly and do not form rows. No specific lipids or proteins other than ATP synthase dimers are required for row formation and membrane remodelling. Long rows of ATP synthase dimers are a conserved feature of mitochondrial inner membranes. They are required for cristae formation and a main factor in mitochondrial morphogenesis.
Metadaten
Author:Thorsten Blum, Alexander Hahn, Thomas Meier, Karen M. Davies, Werner KühlbrandtORCiDGND
URN:urn:nbn:de:hebis:30:3-491414
DOI:https://doi.org/10.1073/pnas.1816556116
ISSN:1091-6490
ISSN:0027-8424
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30760595
Parent Title (English):Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Acad. of Sciences
Place of publication:Washington, DC
Contributor(s):David J. DeRosier
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/02/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/02/19
Tag:ATP synthase; electron cryotomography; membrane curvature; mitochondria; subtomogram averaging
Volume:116
Issue:Art. 201816556
Page Number:6
First Page:1
Last Page:6
Note:
Copyright © 2019 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
HeBIS-PPN:446476803
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0