A comprehensive assessment of apigenin as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound

  • Apigenin (4′,5,7-trihydroxyflavone) (Api) is an important component of the human diet, being distributed in a wide number of fruits, vegetables and herbs with the most important sources being represented by chamomile, celery, celeriac and parsley. This study was designed for a comprehensive evaluation of Api as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. In the set experimental conditions, Api presents antiproliferative activity against the A375 human melanoma cell line, a G2/M arrest of the cell cycle and cytotoxic events as revealed by the lactate dehydrogenase release. Caspase 3 activity was inversely proportional to the Api tested doses, namely 30 μM and 60 μM. Phenomena of early apoptosis, late apoptosis and necrosis following incubation with Api were detected by Annexin V-PI double staining. The flavone interfered with the mitochondrial respiration by modulating both glycolytic and mitochondrial pathways for ATP production. The metabolic activity of human dendritic cells (DCs) under LPS-activation was clearly attenuated by stimulation with high concentrations of Api. Il-6 and IL-10 secretion was almost completely blocked while TNF alpha secretion was reduced by about 60%. Api elicited antiangiogenic properties in a dose-dependent manner. Both concentrations of Api influenced tumour cell growth and migration, inducing a limited tumour area inside the application ring, associated with a low number of capillaries.
Author:Alexandra Ghiţu, Anja Schwiebs, Heinfried Hermann RadekeORCiDGND, Stefana Avram, Istvan Zupko, Andrea Bor, Ioana Zinuca Pavel, Cristina Adriana Dehelean, Camelia Oprean, Florina Bojin, Claudia Farcas, Codruta Soica, Oana Duicu, Corina Danciu
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30995771
Parent Title (English):Nutrients
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/04/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/05/02
Tag:A375 human melanoma cell line; angiogenesis; apigenin; apoptosis; mitochondrial bioenergetics and glycolysis; proliferation
Issue:4, Art. 858
Page Number:19
First Page:1
Last Page:19
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0