Platelet-rich fibrin secretome induces three dimensional angiogenic activation in vitro

  • Different tissue engineering techniques are used to support rapid vascularisation. A novel technique is the use of platelet-rich fibrin (PRF), an autologous source of growth factors. This study was the first to investigate the influence of PRF matrices, isolated following different centrifugation protocols, on human dermal vascular endothelial cells (ECs) in mono-culture and co-culture with human primary fibroblasts (HFs) as an in vitro model for tissue regeneration. Focus was placed on vascular structure formation and growth factor release. HFs and ECs were cultivated with PRF prepared using a high (710 ×g) or low (44 ×g) relative centrifugation force (RCF) over 14 d. Immunofluorescence staining and immunohistochemistry were used to evaluate the microvascular formation. Cell culture supernatants were collected for evaluation of growth factor release. The results showed a PRF-mediated effect on the induction of angiogenesis in ECs. Microvessel-like structure formation was promoted when ECs were combined with low-RCF PRF as compared to high-RCF PRF or control group. The percentage of vascular lumen area was significantly higher in low-RCF PRF, especially at day 7, which coincided with statistically significantly higher growth factor [vascular endothelial factor (VEGF), transforming growth factor β1 (TGF-β1) and platelet derived growth factor (PDGF)] concentration measured in low-RCF PRF as compared to high-RCF PRF or control group. In conclusion, reducing the RCF according to the low-speed centrifugation concept (LSCC) resulted in increased growth factor release and angiogenic structure formation with EC mono-culture, suggesting that PRF may be a highly beneficial therapeutic tool for tissue engineering applications.

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Author:Carlos Herrera-VizcaínoORCiDGND, Eva Dohle, Sarah al- Maawi, Patrick Booms, Robert Alexander SaderORCiDGND, Charles James Kirkpatrick, Joseph Choukroun, Shahram GhanaatiORCiDGND
Pubmed Id:
Parent Title (English):European cells & materials
Publisher:Univ. of Wales
Place of publication:Aberystwyth, Wales
Contributor(s):Mauro Alini
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/04/09
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/05/06
Tag:Platelet-rich fibrin; angiogenesis; low speed centrifugation concept; secretome
Page Number:15
First Page:250
Last Page:264
Copyright policy: This article is distributed in accordance with Creative Commons Attribution Licence (
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung-Weitergabe unter gleichen Bedingungen