3D investigation shows walls and wall-like structures around human germinal centres, probably regulating T- and B-cell entry and exit

  • This study deals with 3D laser investigation on the border between the human lymph node T-zone and germinal centre. Only a few T-cells specific for antigen selected B-cells are allowed to enter germinal centres. This selection process is guided by sinus structures, chemokine gradients and inherent motility of the lymphoid cells. We measured gaps and wall-like structures manually, using IMARIS, a 3D image software for analysis and interpretation of microscopy datasets. In this paper, we describe alpha-actin positive and semipermeable walls and wall-like structures that may hinder T-cells and other cell types from entering germinal centres. Some clearly defined holes or gaps probably regulate lymphoid traffic between T- and B-cell areas. In lymphadenitis, the morphology of this border structure is clearly defined. However, in case of malignant lymphoma, the wall-like structure is disrupted. This has been demonstrated exemplarily in case of angioimmunoblastic T-cell lymphoma. We revealed significant differences of lengths of the wall-like structures in angioimmunoblastic T-cell lymphoma in comparison with wall-like structures in reactive tissue slices. The alterations of morphological structures lead to abnormal and less controlled T- and B-cell distributions probably preventing the immune defence against tumour cells and infectious agents by dysregulating immune homeostasis.

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Author:Miguel Thomos, Patrick WurzelORCiD, Sonja ScharfORCiDGND, Ina KochORCiD, Martin-Leo HansmannGND
Parent Title (English):PLoS ONE
Place of publication:San Francisco, California, US
Document Type:Article
Date of Publication (online):2020/11/10
Date of first Publication:2020/11/10
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2021/08/17
Issue:11, art. e0242177
Page Number:11
First Page:1
Last Page:11
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0