Genetic code expansion facilitates position-selective modification of nucleic acids and proteins

  • Transcription and translation obey to the genetic code of four nucleobases and 21 amino acids evolved over billions of years. Both these processes have been engineered to facilitate the use of non-natural building blocks in both nucleic acids and proteins, enabling researchers with a decent toolbox for structural and functional analyses. Here, we review the most common approaches for how labeling of both nucleic acids as well as proteins in a site-selective fashion with either modifiable building blocks or spectroscopic probes can be facilitated by genetic code expansion. We emphasize methodological approaches and how these can be adapted for specific modifications, both during as well as after biomolecule synthesis. These modifications can facilitate, for example, a number of different spectroscopic analysis techniques and can under specific circumstances even be used in combination.

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Author:Diana Müller, Sven Trucks, Harald SchwalbeORCiDGND, Martin HengesbachORCiDGND
Parent Title (English):ChemPlusChem
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2020/05/22
Date of first Publication:2020/05/22
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/03/08
Tag:fluorescence; genetic code expansion; non-canonical amino acid; non-natural nucleotide; site-specific labeling
Page Number:11
First Page:1233
Last Page:1243
Experiments were supported by DFG through collaborative research center 902: Molecular principles of RNA-based regulation and individual grant SCHW701/22-1. BMRZ is supported by the state of Hesse.
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0