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Adh4, an alcohol dehydrogenase controls alcohol formation within bacterial microcompartments in the acetogenic bacterium Acetobacterium woodii

  • Acetobacterium woodii utilizes the Wood‐Ljungdahl pathway for reductive synthesis of acetate from carbon dioxide. However, A. woodii can also perform non‐acetogenic growth on 1,2‐propanediol (1,2‐PD) where instead of acetate, equal amounts of propionate and propanol are produced as metabolic end products. Metabolism of 1,2‐PD occurs via encapsulated metabolic enzymes within large proteinaceous bodies called bacterial microcompartments. While the genome of A. woodii harbours 11 genes encoding putative alcohol dehydrogenases, the BMC‐encapsulated propanol‐generating alcohol dehydrogenase remains unidentified. Here, we show that Adh4 of A. woodii is the alcohol dehydrogenase required for propanol/ethanol formation within these microcompartments. It catalyses the NADH‐dependent reduction of propionaldehyde or acetaldehyde to propanol or ethanol and primarily functions to recycle NADH within the BMC. Removal of adh4 gene from the A. woodii genome resulted in slow growth on 1,2‐PD and the mutant displayed reduced propanol and enhanced propionate formation as a metabolic end product. In sum, the data suggest that Adh4 is responsible for propanol formation within the BMC and is involved in redox balancing in the acetogen, A. woodii.

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Author:Nilanjan Pal ChowdhuryORCiDGND, Jimyung MoonORCiD, Volker MüllerORCiD
URN:urn:nbn:de:hebis:30:3-572281
DOI:https://doi.org/10.1111/1462-2920.15340
ISSN:1462-2920
ISSN:1462-2912
Parent Title (German):Environmental Microbiology 23.2021, S. 499-511, doi:10.1111/1462-2920.15340, ISSN 1462-2920
Publisher:John Wiley & Sons Ltd.
Place of publication:Oxford [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2020/12/10
Date of first Publication:2020/12/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/02/18
Volume:23.2021
Page Number:13
First Page:499
Last Page:511
HeBIS-PPN:477820743
Institutes:Biowissenschaften / Biowissenschaften
Fachübergreifende Einrichtungen / Biodiversität und Klima Forschungszentrum (BiK-F)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0