Integrity of dural closure after autologous platelet rich fibrin augmentation: an in vitro study
- Background: Watertight closure of the dura mater is fundamental in neurosurgery. Besides the classical suturing techniques, a variety of biomaterials have been proposed as sealants. Platelet rich fibrin (PRF) is an autologous biomaterial which can readily be obtained through low-speed centrifugation of patient’s own blood. It is rich in fibrin, growth factors, leucocytes and cytokines and has shown adhesive properties while promoting the physiological wound healing process. In this study, we investigated the effect of applying PRF in reinforcing the watertight dura mater closure. Methods: We created an in vitro testing device, where the watertight dura mater closure could be hydrostatically assessed. On 26 fresh harvested bovine dura maters, a standardised 20-mm incision was closed with a running suture, and the leak pressure was measured first without (primary leak pressure) and then with PRF augmentation (secondary leak pressure). The two groups of measurements have been statistically analysed with the Student’s paired t test. Results: The “running suture only group” had a leak pressure of 10.5 ± 1.2 cmH2O (mean ± SD) while the “PRF-augmented group” had a leak pressure of 47.2 ± 2.6 cm H2O. This difference was statistically significant (p < 0.001; paired t test). Conclusions: Autologous platelet rich fibrin augmentation reliably reinforced watertight closure of the dura mater to a > 4-fold increased leak pressure after failure of the initial standard running suture technique.
Author: | Ioannis Vasilikos, Jürgen Beck, Shahram Michael GhanaatiORCiDGND, Jürgen Grauvogel, Themistoklis Nisyrios, Konstantinos Grapatsas, Ulrich Hubbe |
---|---|
URN: | urn:nbn:de:hebis:30:3-627337 |
DOI: | https://doi.org/10.1007/s00701-020-04254-4 |
ISSN: | 0942-0940 |
Parent Title (English): | Acta neurochirurgica |
Publisher: | Springer |
Place of publication: | Wien [u.a.] |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2020/02/07 |
Date of first Publication: | 2020/02/07 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2022/05/18 |
Tag: | Autologous biomaterial; Dural onlays; Neurosurgery; Watertight Dural closure |
Volume: | 162 |
Issue: | 4 |
Page Number: | 7 |
First Page: | 737 |
Last Page: | 743 |
Note: | A Correction to this article was published on 03 September 2021. DOI: 10.1007/s00701-021-04907-y |
Note: | Open Access funding enabled and organized by Projekt DEAL. |
HeBIS-PPN: | 496077759 |
Institutes: | Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |