Complementary structures of the yeast phosphate transporter Pho90 provide insights into its transport mechanism
- Highlights • Cryo-EM structures of the yeast low-affinity phosphate importer ScPho90 • Complementary structures reveal insights into the substrate translocation mechanism • Comparisons with homologous transporters highlight the conserved transport mechanism • Regulation by the SPX domain is discussed Summary Phosphate homeostasis is essential for all living organisms. Low-affinity phosphate transporters are involved in phosphate import and regulation in a range of eukaryotic organisms. We have determined the structures of the Saccharomyces cerevisiae phosphate importer Pho90 by electron cryomicroscopy in two complementary states at 2.3 and 3.1 Å resolution. The symmetrical, outward-open structure in the presence of phosphate indicates bound substrate ions in the binding pocket. In the absence of phosphate, Pho90 assumes an asymmetric structure with one monomer facing inward and one monomer facing outward, providing insights into the transport mechanism. The Pho90 transport domain binds phosphate ions on one side of the membrane, then flips to the other side where the substrate is released. Together with functional experiments, these complementary structures illustrate the transport mechanism of eukaryotic low-affinity phosphate transporters.
Author: | Simon SchneiderORCiD, Werner KühlbrandtORCiDGND, Özkan YildizORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-855220 |
DOI: | https://doi.org/10.1016/j.str.2024.04.005 |
ISSN: | 0969-2126 |
Parent Title (English): | Structure |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2024/04/29 |
Date of first Publication: | 2024/04/29 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/05/02 |
Tag: | Cryo-EM; membrane protein; phosphate homeostasis; phosphate translocation mechanism; phosphate transport regulation; phosphate transporter; secondary transporter |
Volume: | 32 |
Issue: | In Press, Corrected Proof |
Page Number: | 27 |
First Page: | 1 |
Last Page: | 10 |
HeBIS-PPN: | 520377575 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | ![]() |