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Light-induced helix movements in channelrhodopsin-2

  • Channelrhodopsin-2 (ChR2) is a cation-selective light-gated channel from Chlamydomonas reinhardtii (Nagel G, Szellas T, Huhn W, Kateriya S, Adeishvili N, Berthold P, et al. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc Natl Acad Sci USA 2003;100:13940-5), which has become a powerful tool in optogenetics. Two-dimensional crystals of the slow photocycling C128T ChR2 mutant were exposed to 473 nm light and rapidly frozen to trap the open state. Projection difference maps at 6Å resolution show the location, extent and direction of light-induced conformational changes in ChR2 during the transition from the closed state to the ion-conducting open state. Difference peaks indicate that transmembrane helices (TMHs) TMH2, TMH6 and TMH7 reorient or rearrange during the photocycle. No major differences were found near TMH3 and TMH4 at the dimer interface. While conformational changes in TMH6 and TMH7 are known from other microbial-type rhodopsins, our results indicate that TMH2 has a key role in light-induced channel opening and closing in ChR2.

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Metadaten
Author:Maria Müller, Christian BamannORCiDGND, Ernst BambergGND, Werner KühlbrandtORCiDGND
URN:urn:nbn:de:hebis:30:3-372187
DOI:https://doi.org/10.1016/j.jmb.2014.11.004
ISSN:1089-8638
ISSN:0022-2836
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25451024
Parent Title (English):Journal of molecular biology
Publisher:Elsevier
Place of publication:Amsterdam [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2014/11/09
Date of first Publication:2014/11/09
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2015/03/30
Tag:channelrhodopsin; conformational changes; electron cryo-microscopy; light-gated ion channel; microbial rhodopsin
Volume:427
Issue:2
Page Number:9
First Page:341
Last Page:349
Note:
© 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by/3.0/).
HeBIS-PPN:368982319
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0