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Phospho-rasputin stabilization by Sec16 is required for stress granule formation upon amino acid starvation

  • Most cellular stresses induce protein translation inhibition and stress granule formation. Here, using Drosophila S2 cells, we investigate the role of G3BP/Rasputin in this process. In contrast to arsenite treatment, where dephosphorylated Ser142 Rasputin is recruited to stress granules, we find that, upon amino acid starvation, only the phosphorylated Ser142 form is recruited. Furthermore, we identify Sec16, a component of the endoplasmic reticulum exit site, as a Rasputin interactor and stabilizer. Sec16 depletion results in Rasputin degradation and inhibition of stress granule formation. However, in the absence of Sec16, pharmacological stabilization of Rasputin is not enough to rescue the assembly of stress granules. This is because Sec16 specifically interacts with phosphorylated Ser142 Rasputin, the form required for stress granule formation upon amino acid starvation. Taken together, these results demonstrate that stress granule formation is fine-tuned by specific signaling cues that are unique to each stress. These results also expand the role of Sec16 as a stress response protein.
Metadaten
Author:Angelica Aguilera-Gomez, Margarita Zacharogianni, Marinke M. van Oorschot, Heide Marika Genau, Rianne Grond, Tineke Veenendaal, Kristina S. Sinsimer, Elizabeth R. Gavis, Christian Behrends, Catherine Rabouille
URN:urn:nbn:de:hebis:30:3-450726
DOI:https://doi.org/10.1016/j.celrep.2017.06.042
ISSN:2211-1247
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28746877
Parent Title (English):Cell Reports
Publisher:Cell Press
Place of publication:Maryland Heights, MO
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/08/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/12/21
Volume:20
Issue:4
Page Number:15
First Page:935
Last Page:948
Note:
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
HeBIS-PPN:426718534
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0