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1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5′-UTR of SARS-CoV-2

  • The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5′-untranslated region (5′-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5′-UUUCGU-3′ hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive 1H, 13C and 15N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.

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Author:Robbin SchniedersGND, Stephen PeterORCiDGND, Elnaz Banijamali, Magdalena RiadORCiD, Nadide AltınçekiçORCiDGND, Jasleen Kaur BainsORCiDGND, Betül Ceylan, Boris FürtigORCiDGND, J. Tassilo GrünORCiDGND, Martin HengesbachORCiDGND, Katharina Felicitas HohmannGND, Daniel Hymon, Božana KnežićGND, Andreas Oxenfarth, Katja PetzoldORCiD, Nusrat QureshiORCiDGND, Christian RichterORCiDGND, Judith Schlagnitweit, Andreas SchlundtORCiDGND, Harald SchwalbeORCiDGND, Elke Stirnal, Alexey Sudakov, Jennifer VögeleORCiD, Anna WackerORCiDGND, Julia WeigandORCiDGND, Julia Wirmer-BartoschekORCiD, Jens WöhnertORCiDGND
URN:urn:nbn:de:hebis:30:3-628372
DOI:https://doi.org/10.1007/s12104-021-10007-w
ISSN:1874-270X
Parent Title (English):Biomolecular NMR assignments
Publisher:Springer Netherlands
Place of publication:Dordrecht [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2021/01/23
Date of first Publication:2021/01/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/01/31
Tag:5′-UTR; COVID19-NMR; SARS-CoV-2; SL5a; Solution NMR spectroscopy
Volume:15
Issue:1
Page Number:9
First Page:203
Last Page:211
Note:
Work at BMRZ is supported by the state of Hesse. Work in Covid19-nmr was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of state of Hesse, the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). Open Access funding enabled and organized by Projekt DEAL.
HeBIS-PPN:507023463
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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