- Essentials
• The role of platelet IL-1β release in chronic inflammation is currently unclear.
• Platelets from 65 patients with varying degrees of chronic inflammation were studied.
• Chronic inflammation linked to reduced levels of intracellular IL-1β and IL-1β release.
• Chronic inflammation induces a phenotype that indicates chronic IL-1β release from platelets.
Abstract
Background: Chronic inflammation is a cardiovascular risk factor, and interleukin-1β (IL-1β) is central to the inflammatory host response. Platelets contain the NLRP3 inflammasome and are able to translate IL-1β messenger RNA (mRNA) and secrete mature IL-1β upon activation. However, the role of a chronic inflammatory environment in platelet IL-1β mRNA and protein content remains unclear.
Objectives: The aim of the current study was to investigate intracellular platelet IL-1β and IL-1β mRNA in a chronic inflammatory state.
Methods: Sixty-five patients with stable inflammation (ie, high-sensitivity C-reactive protein within predefined margins in 2 separate measurements) were stratified according to high-sensitivity C-reactive protein levels in low (0.0-0.9 mg/L), medium (1.0-2.9 mg/L), and high (3.0-9.9 mg/L) risk groups. Platelet reactivity as well as platelet IL-1β protein synthesis were studied.
Results: The highest risk group was characterized by a distinct cardiovascular risk profile and approximately 20% higher platelet counts. While platelet reactivity was not different, a reduction in intracellular platelet IL-1β mRNA and IL-1β protein levels was observed in the highest risk group and was linked to decreased platelet size and granularity. This signature suggests a phenotype of chronic IL-1β secretion and could be experimentally phenocopied by stimulation of platelets from healthy volunteers with either TRAP-6 or collagen related peptide (CRP-XL).
Conclusion: Our data suggest a phenotype of chronic IL-1β secretion by platelets in patients with chronic sterile inflammation.
MetadatenAuthor: | Martin BergerORCiDGND, Hendrik Maqua, Katharina Lysaja, Sebastian Frederik MauseGND, Mathew S. Hindle, Khalid NaseemORCiD, Edgar DahlORCiDGND, Thimoteus SpeerORCiDGND, Nikolaus MarxGND, Katharina SchüttGND |
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URN: | urn:nbn:de:hebis:30:3-827842 |
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DOI: | https://doi.org/10.1016/j.rpth.2023.102261 |
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ISSN: | 2475-0379 |
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Parent Title (English): | Research and practice in thrombosis and haemostasis |
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Publisher: | Elsevier |
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Place of publication: | Amsterdam |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2023/12/19 |
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Date of first Publication: | 2023/11/14 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2024/03/25 |
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Tag: | caspase-1; high sensitive C-reactive protein; inflammation; interleukin-1beta; platelets |
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Volume: | 8.2024 |
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Issue: | 1, art. 102261 |
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Article Number: | 102261 |
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Page Number: | 10 |
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HeBIS-PPN: | 520982665 |
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Institutes: | Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0 |
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