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Phage display selection of HIV specific conserved mimotopes with IgG from long-term non-progressors
- Poster presentation Background The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates. Materials and methods The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1. Results After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1. Conclusion Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.
Author: | Michael Humber, Sascha Antoni, Andreas Schreiber, Berta Rodes, Vicente Soriano, Matthias Dittmar, Ursula Dietrich |
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URN: | urn:nbn:de:hebis:30-44519 |
DOI: | https://doi.org/10.1186/1742-4690-2-S1-P29 |
ISSN: | 1742-4690 |
Parent Title (English): | Retrovirology |
Publisher: | BioMed Central |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Year of Completion: | 2005 |
Date of first Publication: | 2005/12/08 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2007/05/22 |
Volume: | 2 |
Issue: | (Suppl 1):P29 |
Page Number: | 1 |
First Page: | 1 |
Last Page: | 1 |
Note: | licensee BioMed Central Ltd. |
Source: | Retrovirology 2005, 2(Suppl 1):P29. - 2005 International Meeting of The Institute of Human Virology, Baltimore, USA. 29 August – 2 September 2005. - doi:10.1186/1742-4690-2-S1-P29 |
HeBIS-PPN: | 188414290 |
Institutes: | Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Licence (German): | Deutsches Urheberrecht |