Redirection of miRNA‐argonaute complexes to specific target sites by synthetic adaptor molecules
- Dysregulation of miRNAs is connected with a multitude of diseases for which antagomirs and miRNA replacement are discussed as therapeutic options. Here, we suggest an alternative concept based on the redirection of RISCs to non‐native target sites. Metabolically stable DNA‐LNA mixmers are used to mediate the binding of RISCs to mRNAs without any direct base complementarity to the presented guide RNA strand. Physical redirection of a dye‐labeled miRNA model and of specific miRNA‐programmed RISC fractions present in HeLa extracts is demonstrated by pull‐down experiments with biotinylated capture oligonucleotides.
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| Author: | Mathias Bolz, Laura Thomas, Ute Scheffer, Elisabeth Kalden, Roland K. Hartmann, Michael GöbelORCiDGND |
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| URN: | urn:nbn:de:hebis:30:3-565141 |
| DOI: | https://doi.org/10.1002/cbdv.202000272 |
| ISSN: | 1612-1880 |
| Parent Title (German): | Chemistry & biodiversity |
| Publisher: | Wiley-VHCA AG |
| Place of publication: | Zürich |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/05/19 |
| Date of first Publication: | 2020/05/19 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/11/09 |
| Tag: | RNA induced silencing complex; RNA recognition; Western blot; argonaute protein; locked nucleic acids; mRNA; pull-down |
| Volume: | 17 |
| Issue: | 7, e2000272 |
| Page Number: | 10 |
| HeBIS-PPN: | 476869277 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
