Redirection of the transcription factor SP1 to AT rich binding sites by a synthetic adaptor molecule
- The ubiquitous transcription factor SP1 binds to a GC rich consensus sequence. Here we describe an adaptor molecule that mediates binding of SP1 to a non-cognate DNA site rich in AT. The adaptor is comprised of a Dervan-type hairpin polyamide with high affinity to an AT rich hexamer duplex. It also carries a 27mer DNA that contains the SP1 consensus sequence. The synthesis and purification of the polyamide-DNA conjugate is reported. Pulldown experiments and western blot analysis demonstrate adaptor mediated binding of SP1 to the hexamer duplex TTGTTA.
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| Author: | Mathias Bolz, Ute Scheffer, Elisabeth Kalden, Michael GöbelORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-626529 |
| DOI: | https://doi.org/10.1002/hlca.202100095 |
| ISSN: | 1522-2675 |
| Parent Title (English): | Helvetica chimica acta |
| Publisher: | Wiley-VCH |
| Place of publication: | New York, NY |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/08/08 |
| Date of first Publication: | 2021/08/08 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2021/12/20 |
| Tag: | DNA conjugate; DNA recognition; adaptor mediated recruitment; hairpin polyamides; protein capture |
| Volume: | 104 |
| Issue: | 9, art. e2100095 |
| Page Number: | 9 |
| First Page: | 1 |
| Last Page: | 9 |
| Note: | Open access funding enabled and organized by Projekt DEAL. |
| HeBIS-PPN: | 491320094 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
