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Structure, assembly and dynamics of H/ACA-Ribonucleoproteins

  • H/ACA-RNPs are involved in RNA guided pseudouridylation of rRNAs and snRNAs. In this thesis I reconstituted active and labeled archaeal as well as eukaryotic H/ACA-RNPs and studied the structural dynamics of complex assembly and pseudouridine formation. Single molecule FRET spectroscopy was used as method of analysis to study structure, assembly and dynamics of these important complexes.

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Author:Gerd Werner Günter Hanspach
URN:urn:nbn:de:hebis:30:3-574468
Place of publication:Frankfurt am Main
Referee:Martin Hengesbach, Harald SchwalbeORCiD
Document Type:Doctoral Thesis
Language:English
Date of Publication (online):2020/12/16
Year of first Publication:2020
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Granting Institution:Johann Wolfgang Goethe-Universität
Date of final exam:2020/12/14
Release Date:2021/01/13
Tag:H/ACA-RNP; Pseudouridine; Pseudouridylation; Ribonucleoproteins; smFRET
Page Number:221
HeBIS-PPN:474638626
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoDeutsches Urheberrecht