High-precision background correction and artifact suppression for ultrafast spectroscopy by quasi-simultaneous measurements in a split-sample cell
- Alternating acquisition of background and sample spectra is often employed in conventional Fourier-transform infrared spectroscopy or ultraviolet–visible spectroscopy for accurate background subtraction. For example, for solvent background correction, typically a spectrum of a cuvette with solvent is measured and subtracted from a spectrum of a cuvette with solvent and solute. Ultrafast spectroscopies, though, come with many peculiarities that make the collection of well-matched, subtractable background and sample spectra challenging. Here, we present a demountable split-sample cell in combination with a modified Lissajous scanner to overcome these challenges. It allows for quasi-simultaneous measurements of background and sample spectra, mitigating the effects of drifts of the setup and maintaining the beam and sample geometry when swapping between background and sample measurements. The cell is moving between subsequent laser shots to refresh the excited sample volume. With less than 45 μl of solution for 150 μm optical thickness, sample usage is economical. Cell assembly is a key step and covered in an illustrated protocol.
Author: | Erhan DenizORCiD, Jan. G. LöfflerORCiDGND, Alexander KondratievORCiD, Asok Rafael ThunORCiD, Yunxia ShenORCiD, Georg WilleORCiDGND, Jens BredenbeckORCiD |
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URN: | urn:nbn:de:hebis:30:3-757553 |
DOI: | https://doi.org/10.1063/5.0079958 |
ISSN: | 1089-7623 |
Parent Title (English): | Review of scientific instruments |
Publisher: | American Institute of Physics |
Place of publication: | [Erscheinungsort nicht ermittelbar] |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/03/01 |
Date of first Publication: | 2022/03/01 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/07/15 |
Tag: | Cell assembly; Fourier transform spectroscopy; Image processing; Optical properties; Proteins; Sensors; Thermal lensing; Thermo optic effects; Ultrafast laser spectroscopy; Visible spectroscopy |
Volume: | 93 |
Issue: | 3, art. 033001 |
Article Number: | 033001 |
Page Number: | 7 |
First Page: | 1 |
Last Page: | 6 |
Note: | Funding: DFG ; BR 3746/4-1 |
Note: | Funding: DFG ; INST 161/722-1 |
HeBIS-PPN: | 520896270 |
Institutes: | Physik |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Sammlungen: | Universitätspublikationen |
Licence (German): | ![]() |