Niko Kohmer, Lisa Eckermann, Boris Böddinghaus, Udo Götsch, Annemarie Berger, Eva Herrmann, Marhild Kortenbusch, Peter Tinnemann, René Gottschalk, Sebastian Hoehl, Sandra Ciesek
- Testing for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by RT-PCR is a vital public health tool in the pandemic. Self-collected samples are increasingly used as an alternative to nasopharyngeal swabs. Several studies suggested that they are sufficiently sensitive to be a useful alternative. However, there are limited data directly comparing several different types of self-collected materials to determine which material is preferable. A total of 102 predominantly symptomatic adults with a confirmed SARS-CoV-2 infection self-collected native saliva, a tongue swab, a mid-turbinate nasal swab, saliva obtained by chewing a cotton pad and gargle lavage, within 48 h of initial diagnosis. Sample collection was unsupervised. Both native saliva and gargling with tap water had high diagnostic sensitivity of 92.8% and 89.1%, respectively. Nasal swabs had a sensitivity of 85.1%, which was not significantly inferior to saliva (p = 0.092), but 16.6% of participants reported they had difficult in self-collection of this sample. A tongue swab and saliva obtained by chewing a cotton pad had a significantly lower sensitivity of 74.2% and 70.2%, respectively. Diagnostic sensitivity was not related to the presence of clinical symptoms or to age. When comparing self-collected specimens from different material, saliva, gargle lavage or mid-turbinate nasal swabs may be considered for most symptomatic patients. However, complementary experiments are required to verify that differences in performance observed among the five sampling modes were not attributed to collection impairment.
MetadatenAuthor: | Niko KohmerORCiDGND, Lisa Eckermann, Boris Böddinghaus, Udo GötschGND, Annemarie BergerGND, Eva HerrmannORCiDGND, Marhild Kortenbusch, Peter TinnemannORCiDGND, René GottschalkORCiDGND, Sebastian HoehlORCiDGND, Sandra CiesekORCiDGND |
---|
URN: | urn:nbn:de:hebis:30:3-802898 |
---|
DOI: | https://doi.org/10.3390/jcm10245751 |
---|
ISSN: | 2077-0383 |
---|
Parent Title (English): | Journal of Clinical Medicine |
---|
Publisher: | MDPI |
---|
Place of publication: | Basel |
---|
Document Type: | Article |
---|
Language: | English |
---|
Date of Publication (online): | 2023/12/08 |
---|
Date of first Publication: | 2021/12/08 |
---|
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
---|
Release Date: | 2023/12/14 |
---|
Tag: | COVID-19; PCR; SARS-CoV-2; gargle lavage; nasal swab; saliva; self-collected samples |
---|
Volume: | 10 |
---|
Issue: | 24, art. 5751 |
---|
Article Number: | 5751 |
---|
Page Number: | 10 |
---|
First Page: | 1 |
---|
Last Page: | 10 |
---|
Note: | This work is based on research funded in part by the German Federal Ministry of Education and Research through project NaFoUniMedCovid19 [B-FAST]—COVIDready (grant 02WRS1621C). |
---|
HeBIS-PPN: | 51655655X |
---|
Institutes: | Medizin |
---|
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Sammlungen: | Universitätspublikationen |
---|
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
---|