The complete mitochondrial genome of Yarrowia lipolytica

  • We here report the complete nucleotide sequence of the 47.9 kb mitochondrial (mt) genome from the obligate aerobic yeast Yarrowia lipolytica. It encodes, all on the same strand, seven subunits of NADH: ubiquinone oxidoreductase (ND1-6, ND4L), apocytochrome b (COB), three subunits of cytochrome oxidase (COX1, 2, 3), three subunits of ATP synthetase (ATP6, 8 and 9), small and large ribosomal RNAs and an incomplete set of tRNAs. The Y. lipolytica mt genome is very similar to the Hansenula wingei mt genome, as judged from blocks of conserved gene order and from sequence homology. The extra DNA in the Y. lipolytica mt genome consists of 17 group 1 introns and stretches of A+Trich sequence, interspersed with potentially transposable GC clusters. The usual mould mt genetic code is used. Interestingly, there is no tRNA able to read CGN (arginine) codons. CGN codons could not be found in exonic open reading frames, whereas they do occur in intronic open reading frames. However, several of the intronic open reading frames have accumulated mutations and must be regarded as pseudogenes. We propose that this may have been triggered by the presence of untranslatable CGN codons. This sequence is available under EMBL Accession No. AJ307410.

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Author:Stefan Kerscher, Gregor Durstewitz, Serge Casaregola, Claude Gaillardin, Ulrich BrandtORCiDGND
Pubmed Id:
Parent Title (English):Comparative and functional genomics
Publisher:Wiley InterScience ; Hindawi
Place of publication:Chichester [u. a.] ; New York, NY
Document Type:Article
Date of Publication (online):2008/11/12
Year of first Publication:2001
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2008/11/12
Tag:CGN codon; NADH dehydrogenase; Yarrowia lipolytica mitochondrial genome complex I NADH: ubiquinoneoxidoreductase; intronic ORF; tRNA
Page Number:11
First Page:80
Last Page:90
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Comparative and Functional Genomics. 2001 April; 2(2): 80–90. ; doi:10.1002/cfg.72 ;
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 3.0