13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides

  • We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose 13C-spins, (2) the (H)CPC-experiment that correlates each phosphorus with the C40 nuclei of adjacent nucleotides via J(C,P) couplings and (3) the (H)CPC-CCH-TOCSY-experiment that correlates the phosphorus nuclei with the respective C10,H10 ribose signals. The experiments were applied to two RNA hairpin structures. The current set of 13C-direct detected experiments allows direct and unambiguous assignment of the majority of the hetero nuclei and the identification of the individual ribose moieties following their sequential assignment. Thus, 13C-direct detected NMR methods constitute useful complements to the conventional 1H-detected approach for the resonance assignment of oligonucleotides that is often hindered by the limited chemical shift dispersion. The developed methods can also be applied to large deuterated RNAs. Keywords: NMR spectroscopy , Direct carbon , detection , RNA

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Author:Christian RichterORCiDGND, Helena Kovacs, Janina BuckGND, Anna WackerORCiDGND, Boris FürtigORCiDGND, Wolfgang Bermel, Harald SchwalbeORCiDGND
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/20544375
Parent Title (English):Journal of biomolecular NMR
Place of publication:Dordrecht
Document Type:Article
Date of Publication (online):2010/11/12
Date of first Publication:2010/06/11
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2010/11/12
Tag:Direct carbon; NMR spectroscopy; RNA; detection
Page Number:11
First Page:259
Last Page:269
Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Source:Journal of Biomolecular NMR, 47(4): S. 259–269 ; doi: 10.1007/s10858-010-9429-5
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs
Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (English):License LogoCreative Commons - Namensnennung-Nicht kommerziell 4.0