A solid supported membrane based electrophysiological study of the escherichia coli NhaA variant E241C
- The dependence of the Escherichia coli Na+H+ antiporter A (EcNhaA) pH sensor mutant E241C on H+ and Na+ concentrations was tested using a solid supported membrane (SSM) based electrophysiological approach. Proteoliposome preparations with right side out (RSO) oriented carriers were used to investigate the passive downhill uptake mode (physiologically the reverse transport mode) at zero membrane potential. Na+ concentration gradients established with a rapid solution exchange acted as the driving force. When a Na+ concentration gradient was established at symmetrical pH, the transport activity of the E241C EcNhaA variant was similar to that of the wildtype EcNhaA, with no shift of the bell-shaped pH dependence, an increase of the KmNa at acidic pH and a decrease of the KmNa at alkaline pH, supporting the model of a competitive binding of Na+ and H+ to a common binding site.
| Author: | Marco Daniel Kokic |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-301034 |
| Place of publication: | Frankfurt am Main |
| Referee: | Klaus FendlerORCiDGND, Werner MänteleORCiDGND |
| Advisor: | Thomas Mager, Lina Hatahet |
| Document Type: | Bachelor Thesis |
| Language: | English |
| Year of Completion: | 2013 |
| Year of first Publication: | 2013 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Granting Institution: | Johann Wolfgang Goethe-Universität |
| Date of final exam: | 2013/05/08 |
| Release Date: | 2014/05/06 |
| Tag: | Biophysical Chemistry; Electrophysiology |
| Page Number: | 30 |
| HeBIS-PPN: | 34131675X |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Sammlung Biologie / Biologische Hochschulschriften (Goethe-Universität) | |
| Licence (German): |  Deutsches Urheberrecht |
