Impact of different spa waters on inflammation parameters in human keratinocyte HaCaT cells

  • Background: The treatment of different skin conditions with spa waters is a long tradition dating back to at least late Hellenism. Interestingly, independent scientific examinations studying the effect of spa waters are scarce. Objective: In the present in vitro study, we compared the effect of culture media supplemented with (a) thermal spa waters (La Roche-Posay, Avène) and (b) two natural mineral drinking waters (Heppinger, Adelholzener) on physiological parameters in HaCaT keratinocytes. Methods: The different medium preparations were investigated with regard to cell proliferation and cell damage. Moreover, the impact on inflammation parameters with and without ultraviolet B (UVB) irradiation was examined. Results: Two popular thermal spring waters were found to suppress cell proliferation and cell damage. Moreover, these waters reversed the induction of interleukin-6, as measured using enzyme-linked immunosorbent assay and promoter transactivation, and the formation of reactive oxygen species after UVB stimulation. Of note, the two natural mineral waters, which are distributed as drinking waters, had some effect on the above-mentioned parameters but to a lesser extent. Conclusion: In summary, our results show that spa waters, and particularly those derived from thermal springs, reduce parameters associated with inflammation. It seems likely that trace elements such as selenium and zinc are critical for the observed effects.

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Author:Nadja Nicole ZöllerORCiDGND, Eva Maria ValeskyORCiDGND, Matthias HofmannGND, Jürgen Bereiter-HahnORCiDGND, August BerndORCiDGND, Roland KaufmannGND, Markus MeissnerORCiDGND, Stefan KippenbergerORCiDGND
Pubmed Id:
Parent Title (English):Annals of dermatology
Place of publication:Seoul
Document Type:Article
Date of Publication (online):2015/12/07
Date of first Publication:2015/12/07
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/01/28
Tag:Inflammation; Interleukin-6; Keratinocytes; Reactive oxygen species; Spa water; Thermal spring water
Page Number:6
First Page:709
Last Page:714
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (English):License LogoCreative Commons - Namensnennung-Nicht kommerziell 4.0