Open Access

Jana Ambrožová

• The investigation is aimed at re-cultivation in the area of the Krušné Hory (Krušné Mountains) in the Czech Republic. In this area, mainly in the town of Most region, there are many coal mines. The re-cultivation by inundation has been used since 1998. In this paper, the toxicity of the Bílina River profile is evaluated and the possible influence of water and sediment quality on biological revival is assessed. Three types of samples were tested - water from the river profile, black mud with oil products odour and sand from the stream center line. These samples were tested by standard acute toxicity tests on green alga Scenedesmus quadricauda strain Greifswald 15, seeds of Sinapis alba and small crustaceans Daphnia magna strain Straus. The acute toxicity bioassays on Daphnia magna did not exhibit any signs of toxicity and it was recommended to use chronic tests on this organism. Also, no inhibitory effects on the growth of Sinapis alba roots were found. A high growth inhibition of the alga Scenedesmus quadricauda was found. In the culture of Scenedesmus quadricauda teratological formations occured.