Hsp70 forms antiparallel dimers stabilized by post-translational modifications to position clients for transfer to Hsp90

  • Protein folding in cells is regulated by networks of chaperones, including the heat shock protein 70 (Hsp70) system, which consists of the Hsp40 cochaperone and a nucleotide exchange factor. Hsp40 mediates complex formation between Hsp70 and client proteins prior to interaction with Hsp90. We used mass spectrometry (MS) to monitor assemblies formed between eukaryotic Hsp90/Hsp70/Hsp40, Hop, p23, and a client protein, a fragment of the glucocorticoid receptor (GR). We found that Hsp40 promotes interactions between the client and Hsp70, and facilitates dimerization of monomeric Hsp70. This dimerization is antiparallel, stabilized by post-translational modifications (PTMs), and maintained in the stable heterohexameric client-loading complex Hsp902Hsp702HopGR identified here. Addition of p23 to this client-loading complex induces transfer of GR onto Hsp90 and leads to expulsion of Hop and Hsp70. Based on these results, we propose that Hsp70 antiparallel dimerization, stabilized by PTMs, positions the client for transfer from Hsp70 to Hsp90.

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Author:Nina MorgnerORCiDGND, Carla Schmidt, Victoria Beilsten-Edmands, Ima-obong Ebong, Nisha A. Patel, Eugenia M. Clerico, Elaine Kirschke, Soumya Daturpalli, Sophie E. Jackson, David Agard, Carol V. Robinson
URN:urn:nbn:de:hebis:30:3-394256
DOI:https://doi.org/10.1016/j.celrep.2015.03.063
ISSN:2211-1247
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25921532
Parent Title (English):Cell reports
Publisher:Cell Press
Place of publication:Maryland Heights, MO
Document Type:Article
Language:English
Date of Publication (online):2015/05/05
Date of first Publication:2015/04/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/02/17
Volume:11
Issue:5
Page Number:12
First Page:759
Last Page:769
Note:
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
HeBIS-PPN:378146912
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0