Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation

  • Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila, maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar (osk). We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3’ UTR in a region adjacent to A-rich sequences. Using Drosophila S2R+ cultured cells we show that this binding site overlaps with a Bruno1 (Bru1) responsive element (BREs) that regulates osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3’ UTR.
Author:Annabelle Cynthia Elisabeth DoldORCiDGND, Hong Han, Niankun Liu, Andrea Hildebrandt, Mirko BrüggemannORCiDGND, Cornelia Rücklé, Heike Hänel, Anke BuschORCiD, Petra BeliORCiDGND, Katharina ZarnackORCiDGND, Julian KönigORCiDGND, Jean-Yves Roignant, Paul Lasko
Pubmed Id:
Parent Title (English):PLoS Genetics
Publisher:Public Library of Science
Place of publication:San Francisco, Calif.
Contributor(s):Jean-René Huynh
Document Type:Article
Year of Completion:2020
Date of first Publication:2020/01/24
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/02/10
Tag:3' UTR; Drosophila melanogaster; Embryos; Messenger RNA; Oocytes; Ovaries; Protein translation; Sequence motif analysis
Issue:(1): e1008581
Page Number:31
First Page:1
Last Page:31
Copyright: © 2020 Dold et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Institutes:Biowissenschaften / Biowissenschaften
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0