An in vitro model for blood brain barrier permeation

  • The ability to permeate accross the blood brain barrier (BBB) is essential for drugs acting on the central nervous system (CNS). Thus, systems that allow rapid and inexpensive screening of the BBB-permeability properties of novel lead compounds are of great importance for speeding up the drug discovery process in the CNS-area. We used immortalized porcine brain microvessel endothelial cells (PBMECICl-2) to develop a model for measurement of blood-brain barrier permeation of CNS active drugs. Investigation of different cell culture conditions showed, that a system using C6 astrocyte glioma conditioned medium and addition of a cyclic AMP analog in combination with a type IV phosphodiesterase inhibitor (R020-1724) leads to cell layers with transendothelial electrical resistance values up to 300 Ω.cm2. Permeability studies with U-[14C]sucroseg ave a permeability coefficient Pe of 3.24 + 0.14 × 10−4 cm/min, which is in good agreement to published values and thus indicates the formation of tight junctions in vitro.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Author:Roland Bauer, Regina Lauer, Birgit Linz, Fritz Pittner, Günter A. Peschek, Gerhard F. Ecker, Peter Friedl, Christian R. NoeGND
Parent Title (English):Scientia pharmaceutica
Publisher:Österr. Apotheker-Verl.-Ges.
Place of publication:Wien
Document Type:Article
Year of Completion:2002
Date of first Publication:2002/11/22
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/05/27
Tag:Blood brain barrier; immortalized porcine brain microvascular endothelial cells; in vitro model
Page Number:6
First Page:317
Last Page:322
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Institutes:Biochemie, Chemie und Pharmazie / Pharmazie
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0