In vivo generated human CAR T cells eradicate tumor cells
- Chimeric antigen receptor (CAR) T cells are in prime focus of current research in cancer immunotherapy. Facilitating CAR T cell generation is among the top goals. We have recently demonstrated direct in vivo generation of human CD19-CAR T cells by targeting CD8+ cells using lentiviral vectors (LVs). The anti-tumor potency of in vivo generated CAR T cells was assessed in human PBMC-transplanted NSG mice carrying i.v. injected CD19+ Nalm-6 tumor cells. A single injection of CD8-targeted LV delivering CD19-CAR was sufficient to completely eliminate the tumor cells from bone marrow and spleen, whereas control animals contained high levels of CD19+ cells. Tumor elimination was due to in vivo generated CAR+ cells. Notably, these were not only composed of T lymphocytes but also included CAR+ natural killer cells (NK and NKT). This is the first demonstration of tumor elimination by in vivo generated human CAR T cells.
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| Author: | Shiwani Agarwal, Tatjana Weidner, Frederic Bastian ThalheimerORCiDGND, Christian BuchholzORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-544335 |
| DOI: | https://doi.org/10.1080/2162402X.2019.1671761 |
| ISSN: | 2162-402X |
| ISSN: | 2162-4011 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/31741773 |
| Parent Title (English): | OncoImmunology |
| Publisher: | Taylor & Franics |
| Place of publication: | Abingdon |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2019 |
| Date of first Publication: | 2019/10/10 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/05/04 |
| Tag: | CAR T cells; Cell therapy; T-cell targeting; in vivo gene delivery; receptor targeting |
| Volume: | 8 |
| Issue: | 12, e1671761 |
| Page Number: | 8 |
| First Page: | e1671761-1 |
| Last Page: | e1671761-7 |
| Note: | This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
| HeBIS-PPN: | 465570070 |
| Institutes: | Biochemie, Chemie und Pharmazie / Pharmazie |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0 |
