Introduction of a new surgical method to improve bone healing in a large bone defect by replacement of the induced membrane by a human decellularized dermis repopulated with bone marrow mononuclear cells in rat

  • The Masquelet technique for the treatment of large bone defects is a two-stage procedure based on an induced membrane. We eliminate the first surgical step by using a decellularized dermal skin graft (Epiflex®) populated with bone marrow mononuclear cells (BMC), as a replacement for the induced membrane. The aim of this study was to demonstrate the feasibility of this technology and provide evidence of equivalent bone healing in comparison to the induced membrane-technique. Therefore, 112 male Sprague–Dawley rats were allocated in six groups and received a 10 mm femoral defect. Defects were treated with either the induced membrane or decellularized dermis, with or without the addition of BMC. Defects were then filled with a scaffold (β-TCP), with or without BMC. After a healing time of eight weeks, femurs were taken for histological, radiological and biomechanical analysis. Defects treated with Epiflex® showed increased mineralization and bone formation predominantly in the transplanted dermis surrounding the defect. No significant decrease of biomechanical properties was found. Vascularization of the defect could be enhanced by addition of BMC. Considering the dramatic reduction of a patient’s burden by the reduced surgical stress and shortened time of treatment, this technique could have a great impact on clinical practice.

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Author:Maximilian LeibleinORCiDGND, Tobias Kolb, Lion Georg ChristianGND, Katrin SchröderORCiDGND, Ceyhan Yaman, Alexander Schaible, Ingo MarziORCiDGND, Dirk HenrichORCiDGND, Maren Carina JankoORCiDGND
Parent Title (English):Materials
Place of publication:Basel
Document Type:Article
Date of Publication (online):2020/06/09
Date of first Publication:2020/06/09
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/06/12
Tag:bone healing; bone marrow mononuclear cells; human decellularized dermis; induced membrane
Page Number:15
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0