Antibiotic resistance and mobile genetic elements in extensively drug-resistant Klebsiella pneumoniae sequence type 147 recovered from Germany

  • Mobile genetic elements (MGEs), especially multidrug-resistance plasmids, are major vehicles for the dissemination of antimicrobial resistance determinants. Herein, we analyse the MGEs in three extensively drug-resistant (XDR) Klebsiella pneumoniae isolates from Germany. Whole genome sequencing (WGS) is performed using Illumina and MinION platforms followed by core-genome multi-locus sequence typing (MLST). The plasmid content is analysed by conjugation, S1-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot experiments. The K. pneumoniae isolates belong to the international high-risk clone ST147 and form a cluster of closely related isolates. They harbour the blaOXA-181 carbapenemase on a ColKP3 plasmid, and 12 antibiotic resistance determinants on an multidrug-resistant (MDR) IncR plasmid with a recombinogenic nature and encoding a large number of insertion elements. The IncR plasmids within the three isolates share a high degree of homology, but present also genetic variations, such as inversion or deletion of genetic regions in close proximity to MGEs. In addition, six plasmids not harbouring any antibiotic resistance determinants are present in each isolate. Our study indicates that genetic variations can be observed within a cluster of closely related isolates, due to the dynamic nature of MGEs. The mobilome of the K. pneumoniae isolates combined with the emergence of the XDR ST147 high-risk clone have the potential to become a major challenge for global healthcare.

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Author:Kyriaki Xanthopoulou, Alessandra Carattoli, Julia Wille, Lena Maria BiehlORCiDGND, Holger Rohde, Fedja FarowskiORCiDGND, Oleg Krut, Laura Villa, Claudia Feudi, Harald SeifertORCiDGND, Paul G. HigginsORCiD
URN:urn:nbn:de:hebis:30:3-569034
DOI:https://doi.org/10.3390/antibiotics9100675
ISSN:2079-6382
Parent Title (English):Antibiotics
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/10/05
Date of first Publication:2020/10/05
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2020/11/13
Tag:Klebsiella pneumoniae; carbapenem resistance; carbapenemase; extensively drug-resistant; long reads; molecular typing; plasmid; whole genome sequencing
Volume:9
Issue:Art. 675
Page Number:13
HeBIS-PPN:475970780
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universit├Ątspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0