Phototemtide A, a cyclic lipopeptide heterologously expressed from Photorhabdus temperata Meg1, shows selective antiprotozoal activity
- A new cyclic lipopeptide, phototemtide A (1), was isolated from Escherichia coli expressing the biosynthetic gene cluster pttABC from Photorhabdus temperata Meg1. The structure of 1 was elucidated by HR‐ESI‐MS and NMR experiments. The absolute configurations of amino acids and 3‐hydroxyoctanoic acid in 1 were determined by using the advanced Marfey's method and comparison after total synthesis of 1, respectively. Additionally, three new minor derivatives, phototemtides B–D (2–4), were identified by detailed HPLC–MS analysis. Phototemtide A (1) showed weak antiprotozoal activity against Plasmodium falciparum, with an IC50 value of 9.8 μm. The biosynthesis of phototemtides A–D (1–4) was also proposed.
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| Author: | Lei ZhaoORCiDGND, Tien Duy Vo, Marcel Kaiser, Helge Björn BodeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-570424 |
| DOI: | https://doi.org/10.1002/cbic.201900665 |
| ISSN: | 1439-7633 |
| Parent Title (German): | ChemBioChem |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2019/12/09 |
| Date of first Publication: | 2019/12/09 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/12/17 |
| Tag: | Photorhabdus; biosynthesis; peptides; structure elucidation; total synthesis |
| Volume: | 21.2020 |
| Issue: | 9 |
| Page Number: | 5 |
| First Page: | 1288 |
| Last Page: | 1292 |
| HeBIS-PPN: | 477029825 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
