Epitopes of naturally acquired and vaccine‐induced anti‐ebola virus glycoprotein antibodies in single amino acid resolution

  • The Ebola virus (EBOV) can cause severe infections in humans, leading to a fatal outcome in a high percentage of cases. Neutralizing antibodies against the EBOV surface glycoprotein (GP) can prevent infections, demonstrating a straightforward way for an efficient vaccination strategy. Meanwhile, many different anti‐EBOV antibodies have been identified, whereas the exact binding epitopes are often unknown. Here, the analysis of serum samples from an EBOV vaccine trial with the recombinant vesicular stomatitis virus‐Zaire ebolavirus (rVSV‐ZEBOV) and an Ebola virus disease survivor, using high‐density peptide arrays, is presented. In this proof‐of‐principle study, distinct IgG and IgM antibodies binding to different epitopes of EBOV GP is detected: By mapping the whole GP as overlapping peptide fragments, new epitopes and confirmed epitopes from the literature are found. Furthermore, the highly selective binding epitope of a neutralizing monoclonal anti‐EBOV GP antibody could be validated. This shows that peptide arrays can be a valuable tool to study the humoral immune response to vaccines in patients and to support Ebola vaccine development.

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Author:Jasmin Heidepriem, Verena KrählingORCiDGND, Christine Dahlke, Timo WolfORCiDGND, Florian Klein, Marylyn M. Addo, Stephan BeckerORCiDGND, Felix F. Löffler
Parent Title (English):Biotechnology journal
Publisher:Wiley‐VCH Verlag GmbH & Co. KGaA
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2020/05/28
Date of first Publication:2020/05/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/12/05
Tag:Ebola virus; epitope mapping; human sera; neutralizing antibodies; recombinant vesicular stomatitis virus‐Zaire ebolavirus
Page Number:8
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0