RITA modulates cell migration and invasion by affecting focal adhesion dynamics

  • RITA, the RBP‐J interacting and tubulin‐associated protein, has been reported to be related to tumor development, but the underlying mechanisms are not understood. Since RITA interacts with tubulin and coats microtubules of the cytoskeleton, we hypothesized that it is involved in cell motility. We show here that depletion of RITA reduces cell migration and invasion of diverse cancer cell lines and mouse embryonic fibroblasts. Cells depleted of RITA display stable focal adhesions (FA) with elevated active integrin, phosphorylated focal adhesion kinase, and paxillin. This is accompanied by enlarged size and disturbed turnover of FA. These cells also demonstrate increased polymerized tubulin. Interestingly, RITA is precipitated with the lipoma‐preferred partner (LPP), which is critical in actin cytoskeleton remodeling and cell migration. Suppression of RITA results in reduced LPP and α‐actinin at FA leading to compromised focal adhesion turnover and actin dynamics. This study identifies RITA as a novel crucial player in cell migration and invasion by affecting the turnover of FA through its interference with the dynamics of actin filaments and microtubules. Its deregulation may contribute to malignant progression.

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Author:Samira Catharina HoockGND, Andreas Hans RitterORCiDGND, Kerstin Steinhäuser, Susanne Roth, Christian Behrends, Franz Oswald, Christine SolbachORCiDGND, Frank LouwenORCiDGND, Nina-Naomi KreisORCiDGND, Juping YuanORCiDGND
Parent Title (English):Molecular Oncology
Publisher:OA Publishing
Place of publication:London
Document Type:Article
Date of Publication (online):2019/07/28
Date of first Publication:2019/07/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/12/23
Tag:FAK; LPP; RITA; focal adhesion; integrin; invasion
Page Number:21
First Page:2121
Last Page:2141
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0