Echovirus-30 infection alters host proteins in lipid rafts at the cerebrospinal fluid barrier In vitro

  • Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.

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Metadaten
Author:Marie Wiatr, Simon Staubach, Ricardo Figueiredo, Carolin Stump-Guthier, Hiroshi Ishikawa, Christian Schwerk, Horst Schroten, Franz-Georg Hanisch, Henriette Rudolph, Tobias Tenenbaum
URN:urn:nbn:de:hebis:30:3-574806
DOI:https://doi.org/10.3390/microorganisms8121958
ISSN:2076-2607
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/33321840
Parent Title (English):Microorganisms
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/12/10
Date of first Publication:2020/12/10
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2021/01/10
Tag:Echovirus-30; HIBCPP cells; blood-cerebrospinal fluid barrier; enterovirus; lipid raft; viral infection
Volume:8
Issue:Article 1958
HeBIS-PPN:477812902
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universit├Ątspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0