Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach

  • During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. During the first decision, the trophectoderm and the inner cell mass are formed. Subsequently, the inner cell mass segregates into the epiblast and the primitive endoderm. Inner cell mass organoids represent an experimental model system, mimicking the second cell fate decision. It has been shown that cells of the same fate tend to cluster stronger than expected for random cell fate decisions. Three major processes are hypothesised to contribute to the cell fate arrangements: (1) chemical signalling; (2) cell sorting; and (3) cell proliferation. In order to quantify the influence of cell proliferation on the observed cell lineage type clustering, we developed an agent-based model accounting for mechanical cell–cell interaction, i.e. adhesion and repulsion, cell division, stochastic cell fate decision and cell fate heredity. The model supports the hypothesis that initial cell fate acquisition is a stochastically driven process, taking place in the early development of inner cell mass organoids. Further, we show that the observed neighbourhood structures can emerge solely due to cell fate heredity during cell division.

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Author:Tim LiebischORCiD, Armin DruskoGND, Biena Mathew ElavunkalGND, Ernst H. K. StelzerORCiDGND, Sabine C. FischerORCiD, Franziska Matthäus
URN:urn:nbn:de:hebis:30:3-575218
DOI:https://doi.org/10.1038/s41598-020-80141-3
ISSN:2045-2322
Parent Title (German):Scientific Reports
Publisher:Nature Publishing Group
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2020
Year of first Publication:2020
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/01/06
Volume:10
Issue:Article 22405
Page Number:11
HeBIS-PPN:47791392X
Institutes:Biowissenschaften / Biowissenschaften
Wissenschaftliche Zentren und koordinierte Programme / Frankfurt Institute for Advanced Studies (FIAS)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0