The low-copy nuclear gene Agt1 as a novel DNA barcoding marker for Bromeliaceae

  • Background: The angiosperm family Bromeliaceae comprises over 3.500 species characterized by exceptionally high morphological and ecological diversity, but a very low genetic variation. In many genera, plants are vegetatively very similar which makes determination of non flowering bromeliads difficult. This is particularly problematic with living collections where plants are often cultivated over decades without flowering. DNA barcoding is therefore a very promising approach to provide reliable and convenient assistance in species determination. However, the observed low genetic variation of canonical barcoding markers in bromeliads causes problems. Result. In this study the low-copy nuclear gene Agt1 is identified as a novel DNA barcoding marker suitable for molecular identification of closely related bromeliad species. Combining a comparatively slowly evolving exon sequence with an adjacent, genetically highly variable intron, correctly matching MegaBLAST based species identification rate was found to be approximately double the highest rate yet reported for bromeliads using other barcode markers. Conclusion. In the present work, we characterize Agt1 as a novel plant DNA barcoding marker to be used for barcoding of bromeliads, a plant group with low genetic variation. Moreover, we provide a comprehensive marker sequence dataset for further use in the bromeliad research community.

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Author:Fabian Bratzel, Sascha Heller, Nadine Cyrannek, Juraj PauleORCiDGND, Elton M. C. Leme, Anna Loreth, Annika Nowotny, Markus Kiefer, Walter Till, Michael H. J. Barfuss, Christian Lexer, Marcus A. Koch, Georg ZizkaORCiDGND
Parent Title (German):BMC Plant Biology
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Year of Completion:2020
Year of first Publication:2020
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/01/04
Tag:Bromeliads; DNA barcoding; Low-copy nuclear gene; Plant collections
Issue:Article number: 111
This article has been updated to add the correct version of additional file 1.
Institutes:Angeschlossene und kooperierende Institutionen / Senckenbergische Naturforschende Gesellschaft
Biowissenschaften / Institut für Ökologie, Evolution und Diversität
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Licence (German):License LogoCreative Commons - Namensnennung 4.0