Nucleoredoxin knockdown in SH-SY5Y cells promotes cell renewal

  • Nucleoredoxin (NXN) is a redox regulator of Disheveled and thereby of WNT signaling. Deficiency in mice leads to cranial dysmorphisms and defects of heart, brain, and bone, suggesting defects of cell fate determination. We used shRNA-mediated knockdown of NXN in SH-SY5Y neuroblastoma cells to study its impact on neuronal cells. We expected that shNXN cells would easily succumb to redox stress, but there were no differences in viability on stimulation with hydrogen peroxide. Instead, the proliferation of naïve shNXN cells was increased with a higher rate of mitotic cells in cell cycle analyses. In addition, basal respiratory rates were higher, whereas the relative change in oxygen consumption upon mitochondrial stressors was similar to control cells. shNXN cells had an increased expression of redox-sensitive heat shock proteins, Hsc70/HSPA8 and HSP90, and autophagy markers suggested an increase in autophagosome formation upon stimulation with bafilomycin and higher flux under low dose rapamycin. A high rate of self-renewal, autophagy, and upregulation of redox-sensitive chaperones appears to be an attractive anti-aging combination if it were to occur in neurons in vivo for which SH-SY5Y cells are a model.
Author:Lucie Valek, Irmgard TegederORCiD
Parent Title (English):Antioxidants
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/03/13
Date of first Publication:2021/03/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/05/26
Tag:SH-SY5Y neuroblastoma cells; autophagy; cell cycle; chaperone-mediated autophagy; hydrogen peroxide; nucleoredoxin; ubiquitin ligase
Issue:3, art. 449
Page Number:16
First Page:1
Last Page:16
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0