Characterization of extracellular vesicles from preconditioned human adipose-derived stromal/stem cells

  • Cell-free therapy using extracellular vesicles (EVs) from adipose-derived mesenchymal stromal/stem cells (ASCs) seems to be a safe and effective therapeutic option to support tissue and organ regeneration. The application of EVs requires particles with a maximum regenerative capability and hypoxic culture conditions as an in vitro preconditioning regimen has been shown to alter the molecular composition of released EVs. Nevertheless, the EV cargo after hypoxic preconditioning has not yet been comprehensively examined. The aim of the present study was the characterization of EVs from hypoxic preconditioned ASCs. We investigated the EV proteome and their effects on renal tubular epithelial cells in vitro. While no effect of hypoxia was observed on the number of released EVs and their protein content, the cargo of the proteins was altered. Proteomic analysis showed 41 increased or decreased proteins, 11 in a statistically significant manner. Furthermore, the uptake of EVs in epithelial cells and a positive effect on oxidative stress in vitro were observed. In conclusion, culture of ASCs under hypoxic conditions was demonstrated to be a promising in vitro preconditioning regimen, which alters the protein cargo and increases the anti-oxidative potential of EVs. These properties may provide new potential therapeutic options for regenerative medicine.
Author:Alec Geßner, Benjamin Florian KochORCiDGND, Kevin KlannORCiDGND, Dominik Christian FuhrmannORCiDGND, Samira Farmand, Ralf SchubertORCiDGND, Christian MünchORCiD, Helmut GeigerGND, Patrick C. BaerORCiDGND
Parent Title (English):International journal of molecular sciences
Publisher:Molecular Diversity Preservation International
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/03/12
Date of first Publication:2021/03/12
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/05/26
Tag:adipose-derived stromal/stem cells; extracellular vesicles; hypoxia; mesenchymal stromal/stem cells; proteomics; renal tubular epithelial cells
Issue:6, art. 2873
Page Number:15
First Page:1
Last Page:15
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0