Acid dentin lysate failed to modulate bone formation in rat calvaria defects

  • Simple Summary: Tooth roots are increasingly applied for bone reconstruction before implant placement. Growth factors stored in the dentin are assumed to enhance bone regeneration, however, the evidence is low. To this aim, collagen membranes were coated with dentin lysates obtained from extracted porcine teeth or remain untreated. The collagen membranes were tested for their capacity to stimulate bone formation in rat calvarial bone defects. After four weeks of healing, micro-computed tomography and histological analyses revealed that dentin lysates coating had no significant impact on the rather strong bone regeneration reaching a nearly complete defect closure even in untreated defects. It can thus be concluded that dentin lysates do not hinder bone regeneration. Conclusions concerning a possible stimulation of bone regeneration by dentin lysates should not be drawn. Abstract: Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since tooth roots undergo creeping substitution similar to bone grafts, it can be hypothesized that osteoclasts release the growth factors stored in the dentin thereby influencing bone formation. To test this hypothesis, collagen membranes were either soaked in acid dentin lysates (ADL) from extracted porcine teeth or serum–free medium followed by lyophilization. Thereafter, these membranes covered standardized 5-mm-diameter critical-size defects in calvarial bone on rats. After four weeks of healing, micro-computed tomography and histological analyses using undecalcified thin ground sections were performed. Micro-computed tomography of the inner 4.5 mm calvaria defects revealed a median bone defect coverage of 91% (CI: 87–95) in the ADL group and 94% (CI: 65–100) in the control group, without significant differences between the groups (intergroup p > 0.05). Furthermore, bone volume (BV) was similar between ADL group (5.7 mm3, CI: 3.4–7.1) and control group (5.7 mm3, CI: 2.9–9.7). Histomorphometry of the defect area confirmed these findings with bone area values amounting to 2.1 mm2 (CI: 1.2–2.6) in the ADL group and 2.0 mm2 (CI: 1.1–3.0) in the control group. Together, these data suggest that acid dentin lysate lyophilized onto collagen membranes failed to modulate the robust bone formation when placed onto calvarial defects.

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Author:Jila Nasirzade, Karol Alí Apaza Alccayhuaman, Zahra Kargarpour, Ulrike Kuchler, Franz Josef Strauss, Layla Panahipour, Carina Kampleitner, Patrick Heimel, Frank SchwarzORCiDGND, Reinhard Gruber
Parent Title (English):Biology
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/03/05
Date of first Publication:2021/03/05
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/10/19
Tag:autogenous tooth roots; bone augmentation; bone regeneration; collagen membranes; guided bone regeneration; rat calvaria defect
Issue:3, art. 196
Page Number:10
First Page:1
Last Page:10
This research project is funded by a grant (19-070) from the Osteology Foundation, Switzerland. J.N. and R.G. are further supported by a grant from the Austrian Science Fund (FWF) (4072-B28). K.A.A.A. is supported by an Osteology Research Scholarship from the Osteology Foundation, Switzer- land. The authors will receive no benefit of any kind either directly or indirectly.
Open Access Funding by the Austrian Science Fund (FWF).
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0