Magnetization transfer to enhance NOE cross-peaks among labile protons: applications to imino–imino sequential walks in SARS-CoV-2-derived RNAs

  • 2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent-driven exchanges to enhance NOE correlations between exchangeable and non-exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross-peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS-CoV-2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.

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Author:Mihajlo Novakovic, Ēriks Kupče, Tali ScherfORCiD, Andreas Oxenfarth, Robbin SchniedersGND, J. Tassilo GrünORCiDGND, Julia Wirmer-BartoschekORCiD, Christian RichterORCiDGND, Harald SchwalbeORCiDGND, Lucio FrydmanORCiD
Parent Title (English):Angewandte Chemie
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2021/03/08
Date of first Publication:2021/03/08
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/08/30
Tag:2D NMR spectroscopy; CEST; NOESY; RNA; SARS-CoV-2
Page Number:8
First Page:11884
Last Page:11891
This work was supported by the EU Horizon 2020 program (FET-OPEN Grant 828946, PATHOS), Israel Science Foundation Grants 965/18 and 3572/20, and the Perlman Family Foundation. HS was supported by the Goethe Corona Funds, EU-supported iNEXT-discovery, and by DFG-funded collaborative research center 902. Work at BMRZ is supported by the state of Hesse. Joint support to L.F., H.S. was given by the German-Israel Foundation (grant G-1501-302). Open access funding enabled and organized by Projekt DEAL.
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0