The actin-modulating protein synaptopodin mediates long-term survival of dendritic spines

  • Large spines are stable and important for memory trace formation. The majority of large spines also contains synaptopodin (SP), an actin-modulating and plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence of SP within large spines could explain their long lifetime. Indeed, using 2-photon time-lapse imaging of SP-transgenic granule cells in mouse organotypic tissue cultures we found that spines containing SP survived considerably longer than spines of equal size without SP. Of note, SP-positive (SP+) spines that underwent pruning first lost SP before disappearing. Whereas the survival time courses of SP+ spines followed conditional two-stage decay functions, SP-negative (SP-) spines and all spines of SP-deficient animals showed single-phase exponential decays. This was also the case following afferent denervation. These results implicate SP as a major regulator of long-term spine stability: SP clusters stabilize spines, and the presence of SP indicates spines of high stability.
Metadaten
Author:Kenrick Yap, Alexander Drakew, Dinko SmilovicORCiD, Michael Rietsche, Mandy H. Paul, Mario Vuksic, Domenico Del Turco, Thomas DellerORCiDGND
URN:urn:nbn:de:hebis:30:3-623671
DOI:https://doi.org/10.7554/eLife.62944
ISSN:2050-084X
Parent Title (English):eLife
Publisher:eLife Sciences Publications
Place of publication:Cambridge
Document Type:Article
Language:English
Date of Publication (online):2020/12/04
Date of first Publication:2020/12/04
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2021/08/03
Volume:9
Issue:art. e62944
Page Number:31
First Page:1
Last Page:31
HeBIS-PPN:486234916
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universit├Ątspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0