3D investigation shows walls and wall-like structures around human germinal centres, probably regulating T- and B-cell entry and exit
- This study deals with 3D laser investigation on the border between the human lymph node T-zone and germinal centre. Only a few T-cells specific for antigen selected B-cells are allowed to enter germinal centres. This selection process is guided by sinus structures, chemokine gradients and inherent motility of the lymphoid cells. We measured gaps and wall-like structures manually, using IMARIS, a 3D image software for analysis and interpretation of microscopy datasets. In this paper, we describe alpha-actin positive and semipermeable walls and wall-like structures that may hinder T-cells and other cell types from entering germinal centres. Some clearly defined holes or gaps probably regulate lymphoid traffic between T- and B-cell areas. In lymphadenitis, the morphology of this border structure is clearly defined. However, in case of malignant lymphoma, the wall-like structure is disrupted. This has been demonstrated exemplarily in case of angioimmunoblastic T-cell lymphoma. We revealed significant differences of lengths of the wall-like structures in angioimmunoblastic T-cell lymphoma in comparison with wall-like structures in reactive tissue slices. The alterations of morphological structures lead to abnormal and less controlled T- and B-cell distributions probably preventing the immune defence against tumour cells and infectious agents by dysregulating immune homeostasis.
Author: | Miguel Thomos, Patrick WurzelORCiD, Sonja ScharfORCiDGND, Ina KochORCiD, Martin-Leo Hansmann |
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URN: | urn:nbn:de:hebis:30:3-623959 |
DOI: | https://doi.org/10.1371/journal.pone.0242177 |
ISSN: | 1932-6203 |
Parent Title (English): | PLoS ONE |
Publisher: | PLOS |
Place of publication: | San Francisco, California, US |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2020/11/10 |
Date of first Publication: | 2020/11/10 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2021/08/17 |
Volume: | 15 |
Issue: | 11, art. e0242177 |
Page Number: | 11 |
First Page: | 1 |
Last Page: | 11 |
HeBIS-PPN: | 487214765 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Medizin |
Licence (German): | ![]() |